Name : Human EphB3 Protein
Product Source :
Recombinant Human EphB3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly34-Leu559.[Accession | P54753]
Molecular Weight :
The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human EphB3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human EphB3 is greater than 95% as determined by SEC-HPLC.
Background :
Although EphB3 expression is down-regulated in colorectal cancer (CRC) cells compared with normal intestinal epithelial cells.EphB3 is down-regulated in CRC compared to normal mucosa. Hypermethylation of CpG island is contributed to downregulation of EphB3 in CRC. EphB3 expression in tumor cells may be a useful prognostic indicator for patients with CRC.
Synonyms :
EK2; ETK2; HEK2; TYRO6; EphB3; AW456895; Cek10; MDK5; Sek4; EC 2.7.10; EC 2.7.10.1
References & Citations :
(1) Xuan Z, Huang J, Gao L, Wang Y, Wang J, Sun Y. Receptor Tyrosine Kinase EphB3: a Prognostic Indicator in Colorectal Carcinoma. Pathol Oncol Res. 2020 Jan;26(1):541-549. doi: 10.1007/s12253-018-0562-x. Epub 2018 Dec 7. PMID: 30535864.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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