Name : Human APCDD1 Protein
Product Source :
Recombinant Human APCDD1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu27-His492.[Accession | Q8J025]
Molecular Weight :
The protein has a predicted MW of 54.4 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human APCDD1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human APCDD1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-APCDD1 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA.
Background :
Adenomatosis polyposis coli downregulated 1 (APCDD1), a negative regulator of Wnt signaling, was examined to understand detailed mechanisms underlying Wnt signaling tooth development. In situ hybridization showed that Apcdd1 was expressed in the condensed mesenchyme at the bud stage, and in the inner enamel epithelium (IEE), including enamel knot (EK) at the cap stage. APCDD1 modulates the gene expression of Wnt- and EK-related signaling molecules at the cap stage of tooth development, and is involved in tooth cusp patterning by modulating the epithelial rearrangement in the IEE.
Synonyms :
Apcdd1; Drapc1; B7323; FP7019; HHS; HTS; HYPT1
References & Citations :
(1) Neupane S, Sohn WJ, Gwon GJ, Kim KR, Lee S, An CH, Suh JY, Shin HI, Yamamoto H, Cho SW, Lee Y, Kim JY. The role of APCDD1 in epithelial rearrangement in tooth morphogenesis. Histochem Cell Biol. 2015 Oct;144(4):377-87. doi: 10.1007/s00418-015-1345-z. Epub 2015 Jul 14. PMID: 26170146.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Carbonic Anhydrase 2 ProteinFormulation
Integrin alpha V beta 3 Proteinsite
Popular categories:
IGFBP-1
Adhesion GPCRs