From each group, four three = 12 tests were performed, and 2 three = six tests have been performed for females. The outcomes in all tables and histograms are presented as mean values regular deviations. Variations between the values had been estimated by Student’s t-tests. 3. Final results three.1. Cholesteryl sulfate MedChemExpress Nanoparticle Characterization The morphology from the AgNPs and their size distribution are presented in Figure 1. The TEM image taken of your drop-cast film of AgNPs dissolved in water shows that the nanoparticles have been mostly spherical and formed small agglomerations. The size of the nanoparticles ranged from eight nm to 20 nm. Much more specifics about AgNP characterization might be discovered in our earlier study [19].3.1. Nanoparticle Characterization The morphology of the AgNPs and their size distribution are presented in Figure 1. The TEM image taken in the drop-cast film of AgNPs dissolved in water shows that the nanoparticles had been primarily spherical and formed small agglomerations. The size with the 14 Nanomaterials 2021, 11, 2992 5 of nanoparticles ranged from eight nm to 20 nm. Much more particulars about AgNP characterization is often located in our earlier study [19].a bFigure 1. TEM image (a) and size histogram (b) of AgNPs nanoparticles. Figure 1. TEM image (a) and size histogram (b) of AgNPs nanoparticles.3.2. Characterization of AgNPs-NADPH tetrasodium salt Cancer spirulina and Determination Concentration of 3.2. Characterization of AgNPs-Spirulina and Determination of theof the Concentration of Nanoparticles Administered to Animals Nanoparticles Administered to AnimalsSpirulina biomass and its biochemical composition. Based on the to identify silver inside the biomass containing AgNPs was analyzed in order performed evaluation, the content material inbiomass contained 64.7 2.four proteins, 4.6 0.6 lipids, 11.5 o thecarbohydrates, the biomass and its biochemical composition. According 0.eight performed 0.26 0.05 contained 64.7 2.four phycobiliproteins. All lipids, 11.5 0.8 analysis, the biomass -carotene, and 12.4 1.1 proteins, 4.6 0.6 biochemical parameters had been 0.26 0.05 the control and 12.4 1.1 phycobiliproteins. All biochemical carbohydrates, on the level of-carotene,biomass (cultivated devoid of the addition of nanoparticles) (see Table the level of the handle biomass (cultivated without the need of the was 51 /g. parameters were onS1). The content material of silver in the biomass determined by NAAaddition of As a result, in Table receive of silver, each and every animal was administrated with NAA nanoparticles) (seeorder toS1). The1content of silver in the biomass determined by20 mg of AgNPs-Spirulina. Animals from the positive manage group had been administrated 20 mg of was 51 /g. As a result, to be able to get 1 of silver, every single animal was administrated with spirulina biomass devoid of nanoparticles. 20 mg of AgNPs-Spirulina. Animals from the positive manage group have been administrated So as to confirm nanoparticle trapping for the spirulina biomass, the protein fraction, 20 mg of spirulina biomass without per gram of biomass, was extracted. The content of silver in which was 0.52 g of protein nanoparticles. In orderprotein fraction was 41 /g,trapping thatthe spirulina biomass, the protein the to confirm nanoparticle which means to about 80.four from the total level of silver fraction, which was 0.52 g in the biomass. The SEM photos and EDAX spectrum obtained forof of 15 was accumulated of protein per gram of biomass, was extracted. The content material 6 the Nanomaterials 2021, 11, x FOR PEER Assessment AgNPs-Spirulina biomass are presented in Figure two. silver within the protein fraction was 41 /g, meanin.