Demonstrated that the majority of putatively transferred transcripts were non-coding RNAs derived from the mir99alet7c cluster (Chromosome 21: LINC00478). The presence of non-coding sequences from this chromosomal region inside the RNA extracted from EVs was confirmed by qPCR. This suggests that these sequences are carried by throphoblast EVs. Summary/Conclusion: In this study, we showed that bioorthogonal RNA labelling chemistry could be utilized for the deciphering trophoblastBackground: M. tuberculosis (Mtb) produces a wide diversity of lipids that modulate host immune responses as pathogen-associated molecular patterns, T-cell antigens or virulence aspects. This one of a kind repertoire has been primarily deciphered by characterizing the structure and properties in the lipids that constitute the bacillus envelope. Nonetheless, yet CCR9 Antagonist MedChemExpress uncharacterized mycobacterial lipids are released from the CA I Inhibitor drug envelope inside vesicles made by the bacillus itself and within exosome-like vesicles released by host cells during infection. Even though the production of vesicles may be a key path by which bacterial lipids interfere with immune effectors beyond the website of infection, the content material of those vesicles in immunomodulatory mycobacterial lipids remains poorly characterized. Whether vesicles shuttle distinct lipid households like uncharacterized ones, if their composition depends on mycobacterial strains virulence or if they differentially regulate immune responses, remains an open question. In this context, we have undertaken to characterize the nature and properties of mycobacterial lipids shuttled inside mycobacterial and host vesicles. Solutions: Working with virulent and attenuated strains, we performed the international analysis of the lipid content material of bacterial and host exosome-like vesicles, thanks to a sensitive Mtb-dedicated high-performance liquid chromatography-mass spectrometry approach permitting the targeted screening of recognized mycobacterial lipids as well as unbiased identification of new molecules. Additionally, working with reporter cell lines we’ve got analysed the capacity of these vesicles to activate pathogen recognition receptors (PRR) recognized to recognize Mtb lipids, for instance TLR2 and C-type lectins. Outcomes: Focusing on recognized lipid families, we highlight that quite a few of the key immunomodulatory mycobacterial lipids (including strain-specific lipids) are present within vesicles but nevertheless show a selective distribution in comparison with their relative abundance in the bacillus envelope. These variations in mycobacterial lipid profiles are accompanied by a differential activation of tested PRR. Summary/Conclusion: Our study offers critical insights in to the biological function of mycobacterial lipids, by means of their trafficking inside extracellular vesicles, in host athogen interactions of the tuberculosis infection. Funding: This function was funded by CNRS, Fondation pour la Recherche M icale.OS27.ExRNA Atlas evaluation gives an exRNA census and reveals six forms of vesicular and non-vesicular exRNA carrier profiles detectable across human body fluids Oscar D. Murillo1; William Thistlethwaite1; Rocco Lucero1; Sai Lakshmi Subramanian1; Neethu Shah1; Andrew R. Jackson1; Joel Rozowsky2; Robert R. Kitchen3; James Diao4; Timur Galeev4; Jonathan Warrell4; Kristina Hanspers5; Anders Riutta5; Alexander Pico5; Roger P. Alexander6; David Galas6; Andrew I. Su7; Louise C. Laurent8; Kendall Jensen9; Matthew Roth1; Mark B. Gerstein10; Aleksandar Milosavljevic1 Department of Molecular H.