State (CirMarina, Italy) for providing propagating material of Corinto Nero (Sangiovese seedless variant). We’re also grateful to Erika Maul from JFK Geilweilerhof (Germany) for supplying Aspirant-false (Gouais seedless variant), Iordan/Iordan seedless and to Pietro Colosi for Corinto Nero samples from Aeolian Islands. Sangiovese seedless Termarina Nera and the pair Termarone/Termarina Rosa had been introduced in collection because of Stefano Meglioraldi and Marisa Fontana, respectively. We finally acknowledge the assistance of Giulia Talucci in establishing embryo rescue cultures, Danila Cuozzo in pollination trials, Cristina Viola in testing pollen viability and germination, Daniel Pedri inside the microscopic observation of seeds, Michela Bernini in lab help, and Pierluigi Magnago in the management of seedlings deriving from emasculated bunches.Authors’ contributions AS and MSG conceived the project, AS, IG, LC, MSG and PMS designed the experiment. AS, CCN, IG, LC, PMS, SL and SR contributed towards the pollination trials and also the phenotypic characterization in the accessions, LC and PMS did the statistical evaluation. AS evaluated the sanitary status in the plant components. PMS carried out the microscopic observation of seeds and seed traces. EG and FC provided the data about pollen viability and germination. IG performed the embryo rescue. AM, AS, CCN, IG, LC, MSG, PMS, PR, SL and SR took element in genotyping the supplies and interpreting the outcomes. CCN and PMS drafted a few sections, when LC was the main contributor in writing the manuscript. AS, CCN, EG, FC, IG, LC, MSG and PMS revised the manuscript. LC ready the final version, which was read and approved by all authors.Funding This operate was performed together with the economic support provided by the Autonomous Province of Trento (Accordo di Programma) and by Fondazione CARITRO (Post-Doc project APIRENIA, int. ref. 2017.0349).Costantini et al. BMC Plant Biology(2021) 21:Page 30 ofAvailability of data and materials All data generated through this study are included inside the article and in its supplementary facts files or are available from the corresponding DNMT3 Purity & Documentation author on reasonable request. BioSample metadata can be identified in the BioSample database (http://www.ncbi.nlm.nih.gov/biosample/) beneath accession numbers SAMN17282791-SAMN17282807. The partial nucleotide sequences of VIT_02s0025g03330, VIT_06s0004g03800, VIT_11s0016g03590, VIT_11s0016g05820 and VIT_14s0083g00910 are obtainable within the GenBank data libraries (https://www.ncbi.nlm.nih.gov/genbank/) below accession numbers MW464209-MW464218. Ethics approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare that they have no competing interests. Author details 1 Study and Innovation Centre, Fondazione Edmund Mach, Through E. Mach 1, 38010 San Michele all’Adige, Italy. CD40 manufacturer 2Center Agriculture Food Environment (C3A), University of Trento, Via. E. Mach 1, 38010 San Michele all’Adige, Italy. three Center for Plant Science Innovation Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA. 4Department of Plant Sciences, University of California, Davis, CA 95616, USA. 5Institute for Sustainable Plant Protection – Analysis Council of Italy, Largo P. Braccini two, 10095 Grugliasco, Italy. Received: 4 September 2020 Accepted: 1 FebruaryReferences 1. De Jong M, Mariani C, Vriezen HW. The part of auxin and gibberellin in tomato fruit set. J Exp Bot. 2009;60:15232. 2. Dau.