hich is similar to that of other Insects 2021, 12, x FOR PEER Evaluation 8 of 19 species studied right here (Supplementary Components, Table S2), indicating that its sequences have been regular transcripts. Thus, the 25 fig wasp transcriptomes were all well-assembled and comparatively complete.Figure 1. Functional characters of 25 fig wasps : (a) the total number of proteins annotated for 25 fig wasps as well as the completeness in the transcriptomes identified for them by BUSCO; (b) proportions of genes annotated by Nr, COG, KAAS, Figure 1. Functional characters wasps. GO, and all datasets for the 25 figof 25 fig wasps : (a) the total number of proteins annotated for 25 fig wasps and also the completeness with the transcriptomes identified for them by BUSCO; (b) proportions of genes annotated by Nr, COG, KAAS, GO, and all datasets3.two. the 25 fig wasps. for HDAC9 Species unigenes Annotated against Public DatabasesBlasting of BLASTP or HMM against protein databases (Nr, Swiss-Prot, KOG, eggNOG, 3.2. Unigenes and GO) against Public 7859 to 52,340 unigenes (cIAP-2 Storage & Stability median: ten,437) were sucPfam, KEEG,Annotated revealed that Databases Blasting of BLASTP or HMM against protein databases (Nr, Swiss-Prot, KOG, eggNOG, Pfam, KEEG, and GO) revealed that 7859 to 52,340 unigenes (median: ten,437) were effectively annotated for 25 fig wasps (Figure 1; Supplementary Materials, Table S2), as well as the proportions of unigenes annotated ranged from 75.99 to 89.53 (median: 84. 70 ). The proportions annotated against Nr and eggNOG were fairly high, withInsects 2021, 12,eight ofcessfully annotated for 25 fig wasps (Figure 1; Supplementary Materials, Table S2), along with the proportions of unigenes annotated ranged from 75.99 to 89.53 (median: 84. 70 ). The proportions annotated against Nr and eggNOG were comparatively higher, with Nr from 71.45 to 85.89 (median: 78.97 ) and eggNOG from 61.03 to 82.72 (median: 76.16 ). The proportion annotated against the PFAM was the third highest (from 61.99 to 74.83 ; median: 69.14 ). The proportions annotated against other databases are offered in Table S2. In total, 17,952 and 21,285 OCGs may be annotated by GO and KEGG, respectively, for downstream enrichment analysis. 3.three. Phylogenetic Relationships The phylogenetic tree generated from 625 single-copy homologous genes was additional integrated into a single final tree with all branches supported by 100 bootstrap support (Figure 2b). All clades of dioecious fig wasps had been monophyletic, when the monoecious fig wasps were polyphyletic, with Eupristina altissima and Platyscapa sp.-F. rumphii branching very first Insects 2021, 12, x FOR PEER Critique plus the other two Platyscapa species within a distinct clade. Here, we considerof 19 9 them as 1 genus clade Eu/Pl.Figure 2. Phylogenetic relationships with gene-family clusters of 25 fig wasps and 7 other insect species: (a) comparison Figure 2. Phylogenetic relationships with gene-family clusters of 25 fig wasps and 7 other insect species: (a) comparison of the numbers of expanded (dark line) and contracted (light line) gene families among wasps (nodes represent the on the numbers of expanded (dark line) and contracted (light line) gene families among 25 fig 25 fig wasps (nodes represent quantity of gene families); (b) the resultant phylogenetic relationships, such as divergence time and the quantity of exthe number of gene households); (b) the resultantbranches and recommendations (huge numbers of contracted gene families arethe quantity of phylogenetic relationships, such as divergence time and shown in panded/