enolic acids was degraded. On the other hand, if the concentration was higher than 400 mg L-1 , the ratio in the mixture of phenolic acid degradation significantly decreased and only five.9 may be removed at the concentration of 1,000 mg L-1 . Additionally, the highest mycelialbiomass was also observed at 400 mg L-1 . As a result, 400 mg L-1 was chosen for the following degradation experiments. The kinetics from the mixture of 5 phenolic acid degradation and strain P5 development in liquid medium are shown in Figure six. Within the handle, all-natural degradation efficiencies of all 5 phenolic acids at 96 h of incubation have been two.7, four.four, 7.two, 5.0, and four.five for ferulic acid, p-hydroxybenzoic acid, vanillic acid, cinnamic acid, and benzoic acid, respectively. Immediately after 84 h of incubation, ferulic acid and p-coumaric acid could not be detected in the strain P5-inoculated culture, and only three.1 p-hydroxybenzoicFrontiers in Microbiology | frontiersin.orgAugust 2021 | Volume 12 | ArticleWang et al.Co-application of Bacteria and FungusFIGURE 7 | EP Modulator Accession impact of distinct treatment options on the illness incidence of Fusarium wilt of cucumber seedlings at 60 days immediately after transplanting. Treatments: CK, without having any microbial therapy; B2, inoculation with Bacillus amyloliquefaciens B2; P5, inoculation with Pleurotus ostreatus P5; B2 + P5, co-inoculation with B. amyloliquefaciens B2 and P. ostreatus P5. Diverse letters above every single bar indicate considerable differences (p 0.05; Duncan’s test) CDK4 Inhibitor Storage & Stability amongst therapies.acid, 6.five vanillic acid, and four.7 benzoic acid had been left in the strain P5-inoculated culture. Following 96 h of incubation, all phenolic acids added in the strain P5-inoculated culture were totally degraded, plus the dry weight of strain P5 mycelia enhanced to 0.89 g dry weight per liter culture.showed the strongest inhibitory impact on FOC population, which was only 1.62 104 copies g-1 soil within the B2 + P5 remedy, corresponding to 83.two reduction compared to control treatment (Figure 8).Soil Allelochemicals Fusarium Wilt Incidents and Plant Seedling GrowthIn the greenhouse pot experiment, the highest illness incidence (56.three ) occurred inside the CK therapy (Figure 7). The application of strains B2 and P5 significantly decreased the disease incidence by 48.1 and 22.2 , respectively, compared to the CK treatment. Nonetheless, the combined therapy B2 + P5 showed the most beneficial control efficacy of Fusarium wilt by 63.0 (Figure 7). The results showed that application of strain B2 or P5 alone was productive for growth promotion in cucumber plants (Table three). Also, the combined treatment B2 + P5 showed drastically stronger growth promotion impact when compared with B2 and P5 remedies (Table 3). Plant dry weight, plant height, root length, and root dry weight with the B2 + P5 treatment elevated by 64.6, 26.four, 37.6, and 53.eight in comparison with the manage, respectively. All inoculation treatment options reduced the contents of phenolic acids within the soil (Table four). In comparison with the CK remedy, the contents of total phenolic acids were significantly (p 0.05) decreased by 18.9, 35.9, and 63.two in B2, P5, and B2 + P5 treatment options, respectively (Table 4).Partnership Amongst Fusarium Wilt Illness Incidence and Soil F. oxysporum f. sp. cucumerinum and Phenolic AcidsCorrelation coefficients have been calculated in between the illness incidence and abundance of FOC and content material of phenolic acidsTABLE 3 | Effects of 4 treatments on cucumber plant growth (60 days following transplantation). Remedy Plant dry weight (g) CK B2 P5 B2 +