Ollection with the plant was completed in January, 2009. The stem-bark was
Ollection from the plant was carried out in January, 2009. The stem-bark was collected from Itak- Ikot Akap village in Ikono Nearby Government Region of Akwa Ibom State. The plant was collected by an Herbalist Mr. Okon Etefie attached to Pharmacognosy Department in the University of Uyo, and identified by a Botanist named Dr (Mrs.) Margret Bassey of S1PR3 Biological Activity Botany Department in the University of Uyo. A voucher specimen (UUH 998), wasNwidu et al., Afr J Tradit Complement Altern Med. (2014) 11(two):257-dx.doi.org/10.4314/ajtcam.v11i2.five deposited in the University Herbarium. The stem-bark was air-dried and powdered. The pulverized plant material were stored at space temperature until utilized. Preparation and extraction of plant components The stem-bark collected was air-dried and pulverized using harmer mill. The powder plant components had been weighed utilizing weighing balance (BG 4000). Five hundred grams with the stem-bark was weighed and immersed in 3 x 500 ml of ethanol (99.8 ) for 72hrs. The soaked extract was shaken twice everyday. The supernatant have been filtered utilizing Whatman filter paper (pore sizes-20-25. The filtrate of ethanol solvent was decreased in volume nearly to dryness within a rotatory PLK3 Storage & Stability evaporator (BUCCHI USA), at 40 oC. The residue from filtration method have been air-dried for 24hrs, and subjected for the exact same procedure for three successive time. After which the extract was dried under a flow of nitrogen till constant weight was obtained. The yield was 43.4 . The extract was stored in an air tight container in a refrigerator till utilised. Prior to pharmacological assay, a sample of extract was dissolved in distilled water and employed for the animal experiments.Finger Print Evaluation The chromatographic fingerprint with the C. lutea stem-bark extract was established working with a Jasco (Tokyo, Japan), liquid chromatograph equipped using a PU-2089, quaternary solvent pump, a MD-2010 PAD, and an AS-2055, autsampler injector with a 20 L sample loop. The analytical column was a Phenomenex Synergi Hydro RP18, (250 four.six mm i.d.; four m), equipped using a Phenomenex security guard column (4.0 two.0 mm i.d.). The mobile phase composition was: water (eluent A), and metanol (eluent B), each containing 0.05 of TFA. The gradient plan was linear beginning with 0 B to one hundred B in 60 min. The flow rate was 1.0 mL/min. EZChrom Elite Information Program computer software (Chromatec, Idstein, Germany) was applied for each the operation of detector and for information processing. The stem-bark extract (2 mg), was dissolved in 2 mL methanol, filtered via a 0.45-m membrane polytetrafluoroethylene (PTFE), filter (Millex), resuspended in 3 mL of water and 20 L was surrendered to HPLC analysis.Phytochemical Analysis The ESE of C. lutea was subjected to qualitative chemical screening employing common process to reveal glycosides, polyphenols and saponins (Trease and Evans, 2001).Elemental evaluation of the plant stem-bark The elemental component of ESE stem-bark of C. lutea was elucidated utilizing the process of Dahlquist Knoll, (1978) as reported for the C. lutea leaf fractions (Nwidu et al., 2012d).Determination of ionic content material of plant stem-bark This determination was carried out by potentiometric titration as previously reported for leaf fractions (Nwidu et al., 2012d).Animals Swiss albino mice weighing among 25-30 g, and adult albino rats (100-150 g), of each sexes had been obtained in the Faculty of Pharmacy Animal Property, University of Uyo, Uyo, Nigeria. Each of the animals had been housed in common cages under laboratory condition in Depa.