Name : Human GLP-1R Protein
Product Source :
Recombinant Human GLP-1R Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminus. It contains Arg24-Glu139.[Accession | P43220]
Molecular Weight :
The protein has a predicted MW of 39.20 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human GLP-1R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human GLP-1R is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human GLP-1R, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-GLP-1R Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
Background :
Glucagon-like peptide-1 (GLP-1) is produced by the gut, stimulates insulin secretion from the pancreatic β-cells, and inhibits glucagon secretion from the α-cells. The GLP-1 receptor (GLP-1R) agonists are used in the treatment of type 2 diabetes (T2DM).
References & Citations :
Doggrell SA. Do glucagon-like peptide-1 receptor (GLP-1R) agonists have potential as adjuncts in the treatment of type 1 diabetes? Expert Opin Pharmacother. 2018 Oct;19(15):1655-1661. doi: 10.1080/14656566.2018.1519547. Epub 2018 Sep 20. PMID: 30234389.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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