Name : Canine GRP-10 proform Protein

Product Source :
Recombinant Canine GRP-10 proform Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala24-Gly147.[Accession | E2QW58]

Molecular Weight :
The protein has a predicted MW of 40.49 kDa. Due to glycosylation, the protein migrates to 41-50 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Canine GRP-10 proform on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Canine GRP-10 proform is greater than 95% as determined by SEC-HPLC.

Background :
Gastrin-releasing peptide (GRP) is a neuropeptide with growth-stimulatory and tumorigenic properties, and neuropeptides have previously been suggested to play a role in the complex cascade of chemical activity associated with periodontal inflammation.

Synonyms :
Gastrin-releasing peptide; GRP

References & Citations :
Choi Y, Heo SC, Kim YN, Joo JY, Hwang JJ, Bae MK, Kim HJ. Gastrin-Releasing Peptide (GRP) Stimulates Osteoclastogenesis in Periodontitis. Cells. 2020 Dec 31;10(1):50. doi: 10.3390/cells10010050. PMID: 33396360; PMCID: PMC7823805.

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