Name : Human GITR/TNFRSF18 Protein
Product Source :
Recombinant Human GITR/TNFRSF18 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln26-Glu161.[Accession | Q9Y5U5-1]
Molecular Weight :
The protein has a predicted MW of 15.4 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95% SEC-HPLC The purity of Human GITR is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human GITR, His Tag at 0.5μg (100μl/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 27.9ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human GITR, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2μg/ml determined by ELISA. SPR Data Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).
Background :
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
References & Citations :
(1)Knee D A , Hewes B , Brogdon J L. Rationale for anti-GITR cancer immunotherapy[J]. European Journal of Cancer, 2016, 67:1-10.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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