Name : Human HGFA Protein (pro form)
Product Source :
Recombinant Human HGFA Protein (pro form) is expressed from HEK293 with His tag at the C-Terminus. It contains Gln36-Ser655.[Accession | Q04756]
Molecular Weight :
The protein has a predicted MW of 67.2 kDa. Due to glycosylation, the protein (pro form) migrates to 85-100 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human HGFA (pro form) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human HGFA (pro form) is greater than 95% as determined by SEC-HPLC.
Background :
Hepatocyte growth factor activator (HGFA) is a serine protease initially identified as a potent activator of hepatocyte growth factor/scatter factor. Hepatocyte growth factor/scatter factor is known to be critically involved in tissue morphogenesis, regeneration, and tumor progression, via its receptor, MET.In vivo, HGFA also activates macrophage-stimulating protein, which has roles in macrophage recruitment and inflammatory processes, cellular survival and wound healing through its receptor, RON.
References & Citations :
(1) Kataoka H, Kawaguchi M. Hepatocyte growth factor activator (HGFA): pathophysiological functions in vivo. FEBS J. 2010 May;277(10):2230-7. doi: 10.1111/j.1742-4658.2010.07640.x. Epub 2010 Apr 9. PMID: 20402763.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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