Name : Human Histone H2A type 3 Protein

Product Source :
Recombinant Human Histone H2A type 3 Protein is expressed from E.coli without tag. It contains Ser2-Lys130.[Accession | Q7L7L0]

Molecular Weight :
The protein has a predicted MW of 13.99 kDa. The protein migrates to 15-20 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human Histone H2A type 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Background :
The family of histone H2A proved that there are a lot of variants associated with cancer development. HIST3H2A is a promising biomarker for predicting prognosis of pancreatic cancer, and it could be a potential therapeutic target. HIST3H2A might regulate the progression of tumor immune in pancreatic cancer through modulating the JAK-STAT pathway. In addition, the role HIST3H2A in pancreatic cancer may be related to DCST1-AS1, HIST1H2B, SLC12A9-AS1.

Synonyms :
H2AC25; H2AW; HIST3H2A

References & Citations :
Zhao M, Dai R. HIST3H2A is a potential biomarker for pancreatic cancer: A study based on TCGA data. Medicine (Baltimore). 2021 Nov 19;100(46):e27598. doi: 10.1097/MD.0000000000027598. PMID: 34797282; PMCID: PMC8601325.

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