Name : APC-equivalent Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein
Product Source :
Recombinant APC-equivalent Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide.[Accession | P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL]
Molecular Weight :
The protein has a predicted MW of 301.2 kDa.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
ELISA Data Immobilized APC-equivalent Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA-G&B2M&Peptide (RIIPRHLQL) Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA (QC Test). Blocking Data Serial dilutions of Anti-LILRB2 Antibody were added into APC-equivalent Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag: Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 62.7 ng/ml.
Background :
HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
Synonyms :
HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
References & Citations :
(1) Krijgsman D, Roelands J, Hendrickx W, Bedognetti D, Kuppen PJK. HLA-G: A New Immune Checkpoint in Cancer? Int J Mol Sci. 2020 Jun 25;21(12):4528. doi: 10.3390/ijms21124528. PMID: 32630545; PMCID: PMC7350262.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
VEGF165 Protein
FOLR3 Protein
Popular categories:
Frizzled-10/CD350
Fluorescent-labeled Recombinant Proteins