Name : Human IDH1 Protein
Product Source :
Recombinant Human IDH1 Protein is expressed from E.coli with His tag at the C-Terminus. It contains Ser2-Leu414.[Accession | O75874]
Molecular Weight :
The protein has a predicted MW of 47.35 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Supplied as 0.22μm filtered solution in 50mM Tris, 200mM NaCl, 1mM DTT, 10% Glycerol (pH 7.5).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IDH1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IDH1 is greater than 95% as determined by SEC-HPLC.
Background :
Isocitrate dehydrogenase (IDH) is a metabolic enzyme that converts isocitrate to α-ketoglutarate (α-KG). Genetic gain-of-function mutations in IDH1 and IDH2 confer a neomorphic activity that allow reduction of α -KG to (R)-2- hydroxyglutarate, the accumulation of which results in the development of cancers like low grade gliomas and leukemia.Isocitrate dehydrogenase 1 (IDH1) is the most frequently mutated metabolic gene in cancer.
Synonyms :
IDH; IDH1; IDPc; PICD; IDP; DCD; IDH; Isocitrate dehydrogenase; IDCD;
References & Citations :
(1) Chen J, Yang J, Cao P. The Evolving Landscape in the Development of Isocitrate Dehydrogenase Mutant Inhibitors. Mini Rev Med Chem. 2016;16(16):1344-1358. doi: 10.2174/1389557516666160609085520. PMID: 27292784.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CD3 epsilon Protein
Animal-Free IL-24 Protein
Popular categories:
Langerin/CD207
RSV G proteins