Name : Cynomolgus IFN gamma/IFNG Protein
Product Source :
Recombinant Cynomolgus IFN gamma/IFNG Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln24-Gln165.[Accession | P63309]
Molecular Weight :
The protein has a predicted MW of 17.72 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus IFN gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus IFN gamma is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus IFN gamma, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 39.4ng/ml determined by ELISA.
Background :
IFN-gamma and one of its receptor subunits, IFNGR1, are translocated to the nucleus, together with STAT1alpha as one macromolecular complex, via the classical importin-dependent pathway.Put IFN-gamma and its receptor subunit, IFNGR1, in direct contact with the promoter region of IFN-gamma-activated genes with associated increased activity, thus suggesting a transcriptional/cotranscriptional role for IFN-gamma/IFNGR1 as well as a possible role in determining the specificity of IFN-gamma action.
Synonyms :
IFN-gamma-R1; CDw119; CD119; IFNGR1; IFNGR; AVP; FLJ45734; IFN-gamma R1; IFN-gR1; type 2; IFNγ
References & Citations :
(1) Ahmed CM, Johnson HM. IFN-gamma and its receptor subunit IFNGR1 are recruited to the IFN-gamma-activated sequence element at the promoter site of IFN-gamma-activated genes: evidence of transactivational activity in IFNGR1. J Immunol. 2006 Jul 1;177(1):315-21. doi: 10.4049/jimmunol.177.1.315. PMID: 16785527.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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