Name : Biotinylated Cynomolgus IFN alpha/beta R2 Protein
Product Source :
Recombinant Biotinylated Cynomolgus IFN alpha/beta R2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Ile27-Lys243.[Accession | EHH53981.1]
Molecular Weight :
The protein has a predicted MW of 27.75 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Cynomolgus IFN alpha/beta R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Cynomolgus IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC. SPR Data Biotinylated Cynomolgus IFN alpha/beta R2, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.13 μM as determined in SPR assay (Biacore T200).
Background :
Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α’s effects on DCs are mediated through miRNAs as well.
Synonyms :
IFN-R-2; IFNAR2; IFNARB; IFNABR; IFN-alpha; IFN-alpha/beta R2; IFNbR2; IFN-R; β receptor 2; β receptor10;
References & Citations :
(1) Sehgal M, Zeremski M, Talal AH, Ginwala R, Elrod E, Grakoui A, Li QG, Philip R, Khan ZK, Jain P. IFN-α-Induced Downregulation of miR-221 in Dendritic Cells: Implications for HCV Pathogenesis and Treatment. J Interferon Cytokine Res. 2015 Sep;35(9):698-709. doi: 10.1089/jir.2014.0211. Epub 2015 Jun 19. PMID: 26090579; PMCID: PMC4560851.Copy
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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