Name : Human Interferon omega-1 Protein
Product Source :
Recombinant Human Interferon omega-1 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Leu22-Ser195.[Accession | P05000]
Molecular Weight :
The protein has a predicted MW of 21.25 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Interferon omega-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Interferon omega-1 is greater than 95% as determined by SEC-HPLC. SPR Data Human IFNAR1, His Tag immobilized on CM5 Chip can bind Human Interferon omega-1, His Tag with an affinity constant of 20.96 μM as determined in SPR assay (Biacore T200).
Background :
Human Interferon omega-1, a type I IFN, also known as IFN-ω1 or IFNW1, it contains a potential N-glycosylation site like human IFN-β. IFN-ω1 binds to the interferon alpha/beta receptor but not to the interferon gamma receptor. All IFN-ω genes analysed to date are devoid of introns. IFN-ω1 has been shown to be well-tolerated in man and to induce reductions of hepatitis C virus RNA levels in a series of human clinical trials.
Synonyms :
Interferon alpha-II-1; IFNW1; IFN-ω1; IFN-omega-1
References & Citations :
(1) Adolf GR. Antigenic structure of human interferon omega 1 (interferon alpha II1): comparison with other human interferons. J Gen Virol. 1987 Jun;68 (Pt 6):1669-76.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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