Name : Human IFN gamma/IFNG Protein
Product Source :
Recombinant Human IFN gamma / IFNG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln24-Gly161.[Accession | P01579]
Molecular Weight :
The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IFN gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IFN gamma is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IFN gamma, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 32.7ng/ml determined by ELISA.
Background :
Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.
Synonyms :
Interferon-gamma; Interferon-γ; interferon; gamma; IFG; IFI; IFN gamma; IFNγ
References & Citations :
(1)Ikeda H, Old LJ, Schreiber RD. The roles of IFN gamma in protection against tumor development and cancer immunoediting. Cytokine Growth Factor Rev. 2002;13(2):95-109. doi:10.1016/s1359-6101(01)00038-7.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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