Name : Mouse IFN alpha 1 Protein
Product Source :
Recombinant Mouse IFN alpha 1 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Cys24-Lys189.[Accession | P01572]
Molecular Weight :
The protein has a predicted MW of 45.88 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse IFN alpha 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SPR Data Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IFN alpha 1, hFc Tag with an affinity constant of 11.96 nM as determined in SPR assay (Biacore T200).
Background :
IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.
Synonyms :
Interferon alpha-1/13;IFN-alpha-1/13;Interferon alpha-D;LeIF D; IFNalpha 1; IFN alpha1;IFNA1;IFN-alpha-1;Interferon alpha 1
References & Citations :
(1)Coomans de Brachène A, Dos Santos RS, Marroqui L, Colli ML, Marselli L, Mirmira RG, Marchetti P, Eizirik DL. IFN-α induces a preferential long-lasting expression of MHC class I in human pancreatic beta cells. Diabetologia. 2018 Mar;61(3):636-640. doi: 10.1007/s00125-017-4536-4. Epub 2018 Jan 5. PMID: 29305625; PMCID: PMC6241216.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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