Name : Human Activin RIIA/ACVR2A Protein
Product Source :
Recombinant Human Activin RIIA/ACVR2A Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala20-Pro135.[Accession | P27037-1]
Molecular Weight :
The protein has a predicted MW of 14.50 kDa. Due to glycosylation, the protein migrates to 30-42 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Activin RIIA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Activin RIIA is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Activin A, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human Activin RIIA, His Tag with the EC50 of 0.23μg/ml determined by ELISA.
Background :
Activin A receptor type 2A (ACVR2A) is a membrane receptor in the transforming growth factor- beta (TGF-β signaling pathway, which is involved in the regulation of cell proliferation, migration, and apoptosis. Loss of ACVR2A has an important role in cancer progression and distant metastasis and may serve as a prognostic marker in patients with colon cancer.
Synonyms :
ACTR-IIA; ACTRIIA; ACVR2A; ACVR2
References & Citations :
Zhuo C, Hu D, Li J, Yu H, Lin X, Chen Y, Zhuang Y, Li Q, Zheng X, Yang C. Downregulation of Activin A Receptor Type 2A Is Associated with Metastatic Potential and Poor Prognosis of Colon Cancer. J Cancer. 2018 Sep 8;9(19):3626-3633. doi: 10.7150/jca.26790. PMID: 30310521; PMCID: PMC6171025.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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