Name : Human IGFBP-3 Protein
Product Source :
Recombinant Human IGFBP-3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly28-Lys291.[Accession | AAH18962]
Molecular Weight :
The protein has a predicted MW of 29.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IGFBP-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human IGFBP-3, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA (QC Test). SPR Data Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).
Background :
Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer.
Synonyms :
IBP3; IGFBP3; IGFBP-3; BP-53
References & Citations :
(1) Cai Q, Dozmorov M, Oh Y. IGFBP-3/IGFBP-3 Receptor System as an Anti-Tumor and Anti-Metastatic Signaling in Cancer. Cells. 2020 May 20;9(5):1261. doi: 10.3390/cells9051261. PMID: 32443727; PMCID: PMC7290346.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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