Name : Human IGFBP-7 Protein
Product Source :
Recombinant Human IGFBP-7 Protein Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Ser27-Leu282.[Accession | Q16270]
Molecular Weight :
The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IGFBP-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human IGFBP-7, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD93, hFc Tag with the EC50 of 0.74μg/ml determined by ELISA.
Background :
IGFBP-7, also known as Mac25/Angiomodulin (AGM), GFBP-rp1, tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide. IGFBP-7 binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
Synonyms :
IGFBP-7; IBP7; AGM; FSTL2; IGFBP-7v; IGFBPRP1; MAC25; PSF; RAMSVPS; TAF
References & Citations :
(1)Li N, Han J, Tang J, Ying Y. IGFBP-7 inhibits the differentiation of oligodendrocyte precursor cells via regulation of Wnt/β-Catenin signaling. J Cell Biochem. 2018;119(6):4742-4750. doi:10.1002/jcb.26654.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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