Name : Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling)
Product Source :
Recombinant Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus. It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).[Accession | Q13261-1(IL-15RA)&P40933-1(IL-15)]
Molecular Weight :
The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per ug by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human IL-15RA&IL-15 (Primary Amine Labeling) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human IL-2 R beta, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, His Tag with the EC50 of 18.5ng/ml determined by ELISA.
Background :
Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
Synonyms :
IL-15 R alpha;CD215;IL15RA;IL-15RA;IL-15R-alpha1;Interleukin-15;IL-15;IL15; Nanrilkefusp alfa
References & Citations :
(1)Nadeau L, Aguer C. Interleukin-15 as a myokine: mechanistic insight into its effect on skeletal muscle metabolism. Appl Physiol Nutr Metab. 2019 Mar;44(3):229-238. doi: 10.1139/apnm-2018-0022. Epub 2018 Sep 6. PMID: 30189147.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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