Name : Biotinylated Human IL-17R alpha/CD217 Protein
Product Source :
Recombinant Biotinylated Human IL-17R alpha/CD217 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Leu33-Trp320.[Accession | Q96F46-1]
Molecular Weight :
The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human IL-17R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human IL-17R alpha is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.94μg/ml determined by ELISA. Immobilized Human IL-17F, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 1.76μg/ml determined by ELISA.
Background :
Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22.
Synonyms :
CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-7R; IL-7R-alpha; ILRA
References & Citations :
(1)Gaffen S L. An overview of IL-17 function and signaling[J]. Cytokine, 2008, 43(3):0-407.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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