Name : Biotinylated Human IL-21 Protein
Product Source :
Recombinant Biotinylated Human IL-21 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus. It contains Gln32-Ser162.[Accession | Q9HBE4-1]
Molecular Weight :
The protein has a predicted MW of 18.30 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in 20mM NaAc, 500mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 500mM NaCl (pH 5.5).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human IL-21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human IL-21R, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-21, His Tag with the EC50 of 2.5ng/ml determined by ELISA.
Background :
Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
Synonyms :
Interleukin-21; IL-21; Za11; CVID11
References & Citations :
(1)Sim GC, Radvanyi L. The IL-2 cytokine family in cancer immunotherapy. Cytokine Growth Factor Rev. 2014 Aug;25(4):377-90. doi: 10.1016/j.cytogfr.2014.07.018. Epub 2014 Aug 1. PMID: 25200249.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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