Name : Mouse Activin RIIB/ACVR2B Protein
Product Source :
Recombinant Mouse Activin RIIB/ACVR2B Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser19-Thr137.[Accession | P27040-1]
Molecular Weight :
The protein has a predicted MW of 14.78 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse Activin RIIB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse Activin RIIB is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse Activin RIIB, His Tag with the EC50 of 22.1ng/ml determined by ELISA.
Background :
ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with Activin And multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).
Synonyms :
Activin receptor type-2B; ACVR2B; Activin RIIB
References & Citations :
Sakamoto K, Kanematsu-Yamaki Y, Kamada Y, Oka M, Ohnishi T, Miwa M, Asami T, Inooka H. Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology. Biochem Biophys Rep. 2017 Jun 10;11:33-39. doi: 10.1016/j.bbrep.2017.06.001. PMID: 28955765; PMCID: PMC5614685.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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