Name : Biotinylated Human IL-25/IL-17E Protein
Product Source :
Recombinant Biotinylated Human IL-25/IL-17E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Tyr33-Gly177.[Accession | Q9H293-1]
Molecular Weight :
The protein has a predicted MW of 19.24 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human IL-25 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human IL-25 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-17RB, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA.
Background :
IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
Synonyms :
IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25
References & Citations :
(1) Xu M, Dong C. IL-25 in allergic inflammation. Immunol Rev. 2017 Jul;278(1):185-191. doi: 10.1111/imr.12558. PMID: 28658555.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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