Name : Biotinylated Mouse IL-23A Protein
Product Source :
Recombinant Biotinylated Mouse IL-23A Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus. It contains Val22-Ala196.[Accession | Q9EQ14]
Molecular Weight :
The protein has a predicted MW of 22.60 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Mouse IL-23A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Mouse IL-23A is greater than 90% as determined by SEC-HPLC.
Background :
The heterodimeric cytokine interleukin-23 (IL-23 or IL23A/IL12B) is produced by dendritic cells and macrophages and promotes the proinflammatory and regenerative activities of T helper 17 (Th17) and innate lymphoid cells. A recent study has reported that IL-23 is also secreted by lung adenoma cells and generates an inflammatory and immune-suppressed stroma.
Synonyms :
Interleukin-23 subunit alpha;IL-23 subunit alpha;IL-23-A;Interleukin-23 subunit p19;IL-23p19;IL23p19;IL23A;P19;SGRF
References & Citations :
Lim KS, Yong ZWE, Wang H, Tan TZ, Huang RY, Yamamoto D, Inaki N, Hazawa M, Wong RW, Oshima H, Oshima M, Ito Y, Voon DC. Inflammatory and mitogenic signals drive interleukin 23 subunit alpha (IL23A) secretion independent of IL12B in intestinal epithelial cells. J Biol Chem. 2020 May 8;295(19):6387-6400. doi: 10.1074/jbc.RA120.012943. Epub 2020 Mar 24. PMID: 32209656; PMCID: PMC7212629.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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