Name : Human ARTN Protein
Product Source :
Recombinant Human ARTN Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Ala108-Gly220.[Accession | Q5T4W7-1]
Molecular Weight :
The protein has a predicted MW of 39.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 4mM HCl (pH 2.8). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCl (pH2.8).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ARTN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ARTN is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human GFRA3, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.
Background :
Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.
Synonyms :
Artemin; Enovin; Neublastin; ARTNE; EVN; ARTN; NBN
References & Citations :
(1) Hezam K, Jiang J, Sun F, Zhang X, Zhang J. Artemin promotes oncogenicity, metastasis and drug resistance in cancer cells. Rev Neurosci. 2018 Jan 26;29(1):93-98. doi: 10.1515/revneuro-2017-0029. PMID: 28937965.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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