Name : Cynomolgus IL-31 Protein
Product Source :
Recombinant Cynomolgus IL-31 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Leu27-Thr163.[Accession | G7PJ78-1]
Molecular Weight :
The protein has a predicted MW of 16.5 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus IL-31 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus IL-31 is greater than 95% as determined by SEC-HPLC. SPR Data Human IL-31 RA, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-31, His Tag with an affinity constant of 0.715 nM as determined in SPR assay (Biacore T200).
Background :
Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.
Synonyms :
Interleukin-31; IL-31;IL31
References & Citations :
(1) Gibbs BF, Patsinakidis N, Raap U. Role of the Pruritic Cytokine IL-31 in Autoimmune Skin Diseases. Front Immunol. 2019 Jun 21;10:1383. doi: 10.3389/fimmu.2019.01383. PMID: 31281316; PMCID: PMC6598004.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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