Name : Biotinylated Human IL-3 Protein
Product Source :
Recombinant Biotinylated Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus. It contains Ala20-Phe152.[Accession | P08700]
Molecular Weight :
The protein has a predicted MW of 16.9 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human IL-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human IL-3 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human IL-3, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 24.4ng/ml determined by ELISA.
Background :
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Synonyms :
IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
References & Citations :
(1)Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis[J]. Science, 2015, 347(6227):1260-1265.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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