Name : Human IL-3 Protein
Product Source :
Recombinant Human IL-3 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala20-Phe152.[Accession | P08700]
Molecular Weight :
The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC. SPR Data Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.276 μM as determined in SPR assay (Biacore T200).
Background :
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Synonyms :
IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
References & Citations :
(1)Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis[J]. Science, 2015, 347(6227):1260-1265.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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