Name : Biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein
Product Source :
Recombinant Biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ile31-Thr205.[Accession | Q13261-1]
Molecular Weight :
The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human IL-15RA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human IL-15RA is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-15 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-15RA, His Tag with the EC50 of 45.9ng/ml determined by ELISA.
Background :
Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
Synonyms :
IL-15 R alpha; CD215; IL-15RA; MGC104179
References & Citations :
(1)Nadeau L, Aguer C. Interleukin-15 as a myokine: mechanistic insight into its effect on skeletal muscle metabolism. Appl Physiol Nutr Metab. 2019 Mar;44(3):229-238. doi: 10.1139/apnm-2018-0022. Epub 2018 Sep 6. PMID: 30189147.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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