Name : Biotinylated Mouse IL-5 Protein
Product Source :
Recombinant Biotinylated Mouse IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Met21-Gly133.[Accession | P04401]
Molecular Weight :
The protein has a predicted MW of 16.0 kDa. Due to glycosylation, the protein migrates to 16 kDa and 20-35 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per ug by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl, 10% Glycerol (pH 7.0).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Mouse IL-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Mouse IL-5 is greater than 95% as determined by SEC-HPLC. SPR Data Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-5, His-Avi Tag with an affinity constant of 0.511 μM as determined in SPR assay (Biacore T200).
Background :
IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).
Synonyms :
Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF
References & Citations :
(1) Hassani M, Koenderman L. Immunological and hematological effects of IL-5(Rα)-targeted therapy: An overview. Allergy. 2018 Oct;73(10):1979-1988. doi: 10.1111/all.13451. Epub 2018 Oct 8. PMID: 29611207; PMCID: PMC6220846.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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