Name : Human IL-7 Protein
Product Source :
Recombinant Human IL-7 Protein is expressed from E.coli without tag. It contains Asp26-His177.[Accession | P13232]
Molecular Weight :
The protein has a predicted MW of 17.5 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 25mM PB, 250mM NaCl (pH 6.8). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 25mM PB, 250mM NaCl (pH 6.8).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-7 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 52.5ng/ml determined by ELISA. SPR Data Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 4.18 nM as determined in SPR assay (Biacore T200). Cell Based Assay Measured in a cell proliferation assay using murine 2E8 cells. The ED50 for this effect is 0.1 – 0.5 ng/mL (QC tested).
Background :
Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.
Synonyms :
IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
References & Citations :
(1)Park JH, Waickman AT, Reynolds J, Castro M, Molina-París C. IL7 receptor signaling in T cells: A mathematical modeling perspective. Wiley Interdiscip Rev Syst Biol Med. 2019;11(5):e1447. doi:10.1002/wsbm.1447
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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