Name : Human IL-18RAP Protein
Product Source :
Recombinant Human IL-18RAP Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Phe20-Arg356.[Accession | O95256-1]
Molecular Weight :
The protein has a predicted MW of 65.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-18RAP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-18RAP is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-18RAP, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-IL-18RAP Antibody, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA.
Background :
lactoferrin (LTF), peptidoglycan recognition protein 1 and IL-18 receptor accessory protein (IL18RAP) remained significantly elevated in peripheral monocytes of hypertensive individuals in a separate validation cohort. Interestingly, IL18RAP expression associated with MAP in a cohort of African Americans..
Synonyms :
ACPL; CD218b; CDw218b; IL-18R-beta; IL-18RAcP; IL-18Rbeta; IL-1R-7; IL-1R7; IL-1RAcPL; IL18RB; IL18RAP; IL-18R-bet; IL-18R-β; IL-18Rβ
References & Citations :
(1)Hedl M, Zheng S, Abraham C. The IL18RAP region disease polymorphism decreases IL-18RAP/IL-18R1/IL-1R1 expression and signaling through innate receptor-initiated pathways. J Immunol. 2014;192(12):5924-5932. doi:10.4049/jimmunol.1302727
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Frizzled-8 Protein
HAS2 Protein
Popular categories:
Estrogen Receptor
IL-17