Name : Mouse IL-18BP Protein
Product Source :
Recombinant Mouse IL-18BP Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Thr29-Ala193.[Accession | Q9Z0M9]
Molecular Weight :
The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse IL-18BP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Mouse IL-18BP, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated IL-18, No Tag with the EC50 of 23.8ng/ml determined by ELISA (QC Test). SPR Data Mouse IL-18BP, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.86 nM as determined in SPR assay (Biacore T200).
Background :
Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.
Synonyms :
IL-18BP; Igifbp
References & Citations :
(1) Zhou T, Damsky W, Weizman OE, McGeary MK, Hartmann KP, Rosen CE, Fischer S, Jackson R, Flavell RA, Wang J, Sanmamed MF, Bosenberg MW, Ring AM. IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy. Nature. 2020 Jul;583(7817):609-614. doi: 10.1038/s41586-020-2422-6. Epub 2020 Jun 24. PMID: 32581358; PMCID: PMC7381364.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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