Name : Human IL-23 alpha&Mouse IL-12 beta Protein
Product Source :
Recombinant Human IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta).[Accession | Q9NPF7(Human IL-23 alpha)&P43432-1(Mouse IL-12 beta)]
Molecular Weight :
The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-23 alpha&Mouse IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.15μg/ml determined by ELISA.
Background :
Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.
Synonyms :
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β
References & Citations :
(1) Esch A, Masiarz A, Mossner S, Moll JM, Grötzinger J, Schröder J, Scheller J, Floss DM. Deciphering site 3 interactions of interleukin 12 and interleukin 23 with their cognate murine and human receptors. J Biol Chem. 2020 Jul 24;295(30):10478-10492. doi: 10.1074/jbc.RA120.013935. Epub 2020 Jun 9. PMID: 32518162; PMCID: PMC7383385.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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