Name : Biotinylated Human IL-17F Protein
Product Source :
Recombinant Biotinylated Human IL-17F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg31-Gln163.[Accession | Q96PD4]
Molecular Weight :
The protein has a predicted MW of 17.5 /14.9 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human IL-17F on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human IL-17F is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-17 R alpha, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-17F, His Tag with the EC50 of 0.46μg/ml determined by ELISA.
Background :
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus.IL-17F is ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover.
Synonyms :
Cytokine ML-1; IL17F; IL-17F; IL24; IL-24; CANDF6; IL-17F; ML1
References & Citations :
(1)Yang X O , Chang S H , Park H , et al. Regulation of inflammatory responses by IL-17F[J]. Journal of Experimental Medicine, 2008, 205(5):1063-1075.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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