Name : Cynomolgus IL-21R Protein
Product Source :
Recombinant Cynomolgus IL-21R Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Cys41-Gly254.[Accession | G7Q0S8]
Molecular Weight :
The protein has a predicted MW of 25.70 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus IL-21R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus IL-21R is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-21 at 2μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus IL-21R, His Tag with the EC50 of 34.3ng/ml determined by ELISA.
Background :
IL-21 and IL-21R were highly expressed in the lesional skin and peripheral blood of psoriasis patients. IL-21 promoted CD4 T cells proliferation and Th17 cells differentiation and inhibiting Treg cells differentiation by upregulating RORγt expression and downregulating Foxp3 expression, with increased expression and secretion of IL-17A and IL-22.Microbial translocation and the associated immune activation during HIV-1 infection may lead to high expression levels of the IL-21R activation marker in RM B cells, a feature associated with increased apoptosis and a reduced number of these cells in the circulation.
Synonyms :
IL-21 receptor; IL-21R; CD360; NILR
References & Citations :
(1) Sammicheli S, Hejdeman B, Rethi B, Chiodi F. Immune activation and increased IL-21R expression are associated with the loss of memory B cells during HIV-1 infection. J Intern Med. 2012 Nov;272(5):492-503. doi: 10.1111/j.1365-2796.2012.02550.x. Epub 2012 Jun 15. PMID: 22530560.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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