Name : Human IL-12 R beta 1/CD212 Protein
Product Source :
Recombinant Human IL-12 R beta 1/CD212 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Cys24-Glu540.[Accession | P42701-1]
Molecular Weight :
The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-12 R beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-12 R beta 1 is greater than 95% as determined by SEC-HPLC. SPR Data Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200).
Background :
Human IL12RB1 is an autosomal gene that is essential for mycobacterial disease resistance and T cell differentiation. Lung and T cell IL12RB1 expression is allele-biased, and the extent to which cells express one IL12RB1 allele is unaffected by activation. Furthermore following its expression the IL12RB1 pre-mRNA is processed into either IL12RB1 Isoform 1 (IL12Rβ1, a positive regulator of IL12 responsiveness) or IL12RB1 Isoform 2 (a protein of heretofore unknown function).
Synonyms :
IL-12R-beta-1; IL-12RB1; IL12R; IL12RB; CD212; IL-12R subunit beta-1; IL-12R-BETA1; MGC34454;IL-12R-β-1; IL-12R subunit β-1; IL-12R-β1
References & Citations :
(1) Reeme AE, Claeys TA, Aggarwal P, Turner AJ, Routes JM, Broeckel U, Robinson RT. Human IL12RB1 expression is allele-biased and produces a novel IL12 response regulator. Genes Immun. 2019 Mar;20(3):181-197. doi: 10.1038/s41435-018-0023-2. Epub 2018 Mar 30. PMID: 29599514; PMCID: PMC6165718.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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