Name : Human IL-31 RA Protein
Product Source :
Recombinant Human IL-31 RA Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala20-Glu519.[Accession | Q8NI17-1]
Molecular Weight :
The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 90-116 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-31 RA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-31 RA is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-31 RA, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-31 RA Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test). SPR Data Human IL-31, His Tag immobilized on CM5 Chip can bind Human IL-31 RA, His Tag with an affinity constant of 82.4nM as determined in a SPR assay (Biacore T200).
Background :
Interleukin-31 receptor A (IL-31RA) is a newly identified type I cytokine receptor, that is related to gp130, the common receptor of the interleukin (IL) -6 family cytokines. IL-31RA forms a functional receptor complex for IL-31 together with the beta subunit of oncostatin M receptor (OSMRbeta). OSMRbeta is expressed in a subset of small-sized nociceptive neurons of adult dorsal root ganglia (DRGs).IL-31 and OSM may thus have redundant functions in the development of OSMRbeta-expressing neurons.
Synonyms :
IL-31RA; IL-31R-alpha; Gp130-like receptor; CRL3; GPL; ZcytoR17; GLM-R; CRL3Glmr; GLM-RMGC125346; PRO21384
References & Citations :
(1) Bando T, Morikawa Y, Komori T, Senba E. Complete overlap of interleukin-31 receptor A and oncostatin M receptor beta in the adult dorsal root ganglia with distinct developmental expression patterns. Neuroscience. 2006 Nov 3;142(4):1263-71. doi: 10.1016/j.neuroscience.2006.07.009. Epub 2006 Aug 22. PMID: 16926070.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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