Name : Biotinylated Human IL-22R alpha 1&IL-10R beta Protein
Product Source :
Recombinant Biotinylated Human IL-22R alpha 1&IL-10R beta Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-Terminus. It contains His16-Thr228(IL-22R alpha 1)&Met20-Ser220(IL-10R beta).[Accession | Q8N6P7(IL-22R alpha 1)&Q08334(IL-10R beta)]
Molecular Weight :
The protein has a predicted MW of 52.07 kDa(IL-22R alpha 1)&48.85 kDa(IL-10R beta). Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human IL-22R alpha 1&IL-10R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human IL-22R alpha 1&IL-10R beta is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-22, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA.
Background :
IL-22Rα1&IL-10Rβ are receptors for IL-22 and IL-10, mediate the activation of JAK-STAT, MAPK and other signaling pathways, and participate in the process of many inflammatory diseases. IL-22 Rα1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL10 Rβ or IL20 Rβ and the respective ligands is required for signal transduction[1].
References & Citations :
[1] Mossner S, Kuchner M, Fazel Modares N, Knebel B, Al-Hasani H, Floss DM, Scheller J. Synthetic interleukin 22 (IL-22) signaling reveals biological activity of homodimeric IL-10 receptor 2 and functional cross-talk with the IL-6 receptor gp130. J Biol Chem. 2020 Aug 28;295(35):12378-12397.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
IL-31 Protein
IL-3 Protein
Popular categories:
Topoisomerase
IL-32