Name : Mouse IL-5 R alpha/CD125 Protein

Product Source :
Recombinant Mouse IL-5 R alpha/CD125 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp18-His339.[Accession | P21183]

Molecular Weight :
The protein has a predicted MW of 37.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl (pH 7.4).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse IL-5 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse IL-5 R alpha is greater than 95% as determined by SEC-HPLC. SPR Data Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-5, His Tag with an affinity constant of 0.495 μM as determined in SPR assay (Biacore T200).

Background :
Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.

Synonyms :
CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560

References & Citations :
(1) Garcia G , Taille C , Laveneziana P , et al. Anti-interleukin-5 therapy in severe asthma[J]. European Respiratory Review, 2013, 22(129):251-257.

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