Name : Human AXL Protein
Product Source :
Recombinant Human AXL Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala26-Pro449.[Accession | P30530-1]
Molecular Weight :
The protein has a predicted MW of 48.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by SEC-HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human AXL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human AXL is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human AXL, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA.
Background :
Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.
Synonyms :
Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1
References & Citations :
(1)Zhu C, Wei Y, Wei X. AXL receptor tyrosine kinase as a promising anti-cancer approach: functions, molecular mechanisms and clinical applications. Mol Cancer. 2019 Nov 4;18(1):153. doi: 10.1186/s12943-019-1090-3. PMID: 31684958; PMCID: PMC6827209.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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