Name : Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Protein
Product Source :
Recombinant Human Integrin alpha X beta 2(ITGAX&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe20-Pro1107(ITGAX) acidic tail & Gln23-Asn700(ITGB2) basic tail.[Accession | P20702-1(ITGAX)&P05107-1(ITGB2)]
Molecular Weight :
The protein has a predicted MW of 125.50 kDa(ITGAX)&79.50 kDa(ITGB2). Due to glycosylation, the protein migrates to 130-135 kDa(ITGAX)&85-95 kDa(ITGB2) kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ITGAX&ITGB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ITGAX&ITGB2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human ITGAX&ITGB2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA.
Background :
The integrin alpha X beta 2 (CD11c/CD18, p150,95) binds ligands through the I domain of the alpha X subunit. Ligands include the complement factor fragment iC3b, a key component in the innate immune defense, which, together with the expression of alpha X beta 2 on dendritic cells and on other leukocytes, suggests a role in the immune response.
Synonyms :
αXβ2; CD11c; SLEB6; CD18; ITGAX&ITGB2; ITGA; ITGB2
References & Citations :
Vorup-Jensen T, Ostermeier C, Shimaoka M, Hommel U, Springer TA. Structure and allosteric regulation of the alpha X beta 2 integrin I domain. Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1873-8. doi: 10.1073/pnas.0237387100. Epub 2003 Jan 28. PMID: 12554829; PMCID: PMC149926.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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