Name : Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein
Product Source :
Recombinant Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe31-Val992(ITGAV) acidic tail&GLy27-Asp718(ITGB3) basic tail.[Accession | P06756-1(ITGAV)&P05106-1(ITGB3)]
Molecular Weight :
The protein has a predicted MW of 114 kDa (ITGAV)&81.3 kDa (ITGB3). Due to glycosylation,the protein migrates to 85-140 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ITGAV&ITGB3 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human ITGAV&ITGB3 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human ITGAV&ITGB3, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Background :
ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling.
Synonyms :
αVβ3; Integrin alpha V beta 3; beta3; alphaV; ITGAV&ITGB3;Integrin alpha V β 3; β3
References & Citations :
(1)Morandi E M , Verstappen R , Zwierzina M E , et al. ITGAV and ITGA5 diversely regulate proliferation and adipogenic differentiation of human adipose derived stem cells[J]. Scientific Reports, 2016, 6(1):28889.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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