Name : Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein
Product Source :
Recombinant Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe31-Val992(ITGAV) acidic tail&Gly24-Asn719(ITGB5) basic tail.[Accession | P06756-1(ITGAV)&P18084(ITGB5)]
Molecular Weight :
The protein has a predicted MW of 114 kDa (ITGAV)&81.4 kDa (ITGB5). Due to glycosylation,the protein migrates to 85-140 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ITGAV&ITGB5 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human ITGAV&ITGB5 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human ITGAV&ITGB5, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA.
Background :
Integrin ITGAV & ITGB5 is a type of integrin that binds to matrix macromolecules and proteinases and thereby stimulates angiogenesis. However, it inhibits angiogenesis. It consists of two components, integrin alpha V and integrin beta 5. Integrin ITGAV & ITGB5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.(Microbial infection) Integrin ITGAV & ITGB5 acts as a receptor for adenovirus type C.
Synonyms :
αVβ5; Integrin alpha V beta 5; beta5; alphaV; ITGAV&ITGB5;Integrin alpha V β 5; β5
References & Citations :
(1)Karasu Ebru E, Nilsson Bo B, Köhl Jörg J, Lambris John D JDFriedlander M , Theesfeld C L , Sugita M , et al. Involvement of integrins alpha v beta 3 and alpha v beta 5 in ocular neovascular diseases.[J]. Proceedings of the National Academy of Sciences, 1996, 93(18):9764-9769.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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