Name : Mouse Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein
Product Source :
Recombinant Mouse Integrin alpha 2 beta 1(ITGA2&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Tyr27-Thr1129(ITGA2) acidic tail & Gln21-Asp728(ITGB1) basic tail.[Accession | Q62469(ITGA2)&P09055-1(ITGB1)]
Molecular Weight :
The protein has a predicted MW of 126.60 kDa(ITGA2)&82.80 kDa(ITGB1). Due to glycosylation, the protein migrates to 128-138 kDa(ITGA2)&105-115 kDa(ITGB1) based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse ITGA2&ITGB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse ITGA2&ITGB1 is greater than 95% as determined by SEC-HPLC.
Background :
Integrin alpha 2 beta 1 is a positive regulator of collagenase (MMP-1) and collagen alpha 1(I) gene expression.HOS cells normally lacking alpha 2 beta 1 integrin were forced to express it, and this prevented the down-regulation in the levels of alpha 1 (I) collagen mRNA when cells were grown inside collagen gels. The data indicate that the level of MMP-1 expression is regulated by the collagen receptor alpha 2 beta 1 integrin.
Synonyms :
α2β1; ITGA2&ITGB1; ITGA2; ITGB1;
References & Citations :
Riikonen T, Westermarck J, Koivisto L, Broberg A, Kähäri VM, Heino J. Integrin alpha 2 beta 1 is a positive regulator of collagenase (MMP-1) and collagen alpha 1(I) gene expression. J Biol Chem. 1995 Jun 2;270(22):13548-52. doi: 10.1074/jbc.270.22.13548. PMID: 7768957.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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